Exit the "analysis window" and go back to the "main window" (refer to menu bar icons image above) 2. Keep the electrodes and muscle transducer attached as they were in the first exercise. 3. Set the "Amp" value to 5 and set the "#p" value to 15. Make sure the frequency "Hz' is set to 1. 4. Remember to "apply" the changes. During this exercise only the frequency "Hz" value will be changed, to demonstrate summation and tetanus. Click "Apply" after each change. 5. In the "Mark" box type "1Hz". Start recording, then press Enter on your keyboard to mark the start of the experiment. Select "Fire" and record until all 15 pulses are done. Select "Stop". 6. Change the stimulus frequency" Hz" to 2 > Select "Apply". Type "2Hz" in the "Mark" box. Start recording > "Fire" > Stop (once all 15 pulses are fired). 7. Repeat step 6, increasing the stimulus frequency to 3, 4, 5, 10, 15, 20 Hz. NOTE: Once you get to 15 and 20 Hz, change the number of pulses "#p" to 50. This needs to be increased to see the effects of summation and tetanus. Remember to select "Apply". 8. Continue to record for a few seconds after 20Hz pulses to see the relaxation of the finger 9. Select "Save as" and save to your USB or X: drive 10. Once the data is saved, select the "Analysis" button. Buttons which will be useful to you during the analysis include the "double display" and "autoscale" buttons 11. Your data at the higher frequencies should look similar to the figure below